Next-Gen Flow
Cytometry & Sorting
Next-Gen flow
Cytometry & Sorting
Technical
Expert
The Next-Gen flow Cytometry & Sorting Core Facility provides advanced services in multiparametric flow cytometry and mass cytometry, supporting experimental design, antibody panel development, training, and data analysis.
Its mission is to enable cutting-edge, high-dimensional single-cell analysis to foster translational and precision research.
Dr. Gloria Olearo
INSTRUMENTS
The facility is equipped with:
- BD FACSymphony™ A5: High-parameter cytometer (5 lasers, 31 detectors) for up to 29-color analysis.
- BD FACSCanto™ II: User-friendly analyzer with HTS option for high-throughput analysis (3 lasers, 10 detectors).
- BD FACSAria™ Fusion: High-performance, biosafety-equipped cell sorter (4-way, 14-color sorting).
- BD Rhapsody™ System: Single-cell capture and barcoding platform for transcriptomic and proteomic profiling.
- Helios™ Mass Cytometer: High-dimensional single-cell analysis using metal-tagged antibodies (up to 45 markers).
- Hyperion™ Imaging System: Mass cytometry-based imaging for spatial profiling in tissues (>1 mm² scan area, >40 markers).
Reserve instruments
To reserve instruments, use the “Agendo” management system: https://upo.agendoscience.com/.
Infrastucture units
The Core Facility is composed of two Infrastructure Units:
1. Multiparametric flow cytometry with cell sorting capabilities and Rhapsody system for single-cell RNA sequencing
The multiparametric flow cytometry unit with cell sorting capabilities enables rapid and precise analysis (via 2 analyzers) and isolation (via 1 cell sorter) of cellular subpopulations. The Rhapsody system for single-cell RNA sequencing allows for the characterization of the transcriptomic profile of individual cells, integrating it with various surface markers through the use of oligonucleotide-conjugated antibodies. This provides unprecedented resolution in the study of cellular heterogeneity.
Scientific supervisor: Dr. Davide Raineri
The Unit offers:
- Multiparametric analysis of up to 30 cellular markers simultaneously.
- Cell sorting (up to 4 populations simultaneously, including plate-based sorting).
- scRNA-seq library preparation using BD Rhapsody technology.
2. Mass cytometry (CyTOF) combined with Hyperion imaging
The mass cytometry unit enables the simultaneous analysis of over 40 parameters in single-cell suspensions. In addition, integration with the Hyperion imaging mass cytometry system allows a high-resolution spatial exploration of tissue architecture, supporting advanced studies of the tissue microenvironment and cell interactions.
Scientific supervisor: Prof. Annalisa Chiocchetti
The Unit offers:
- Cytometric analysis of cell suspensions with over 40 markers.
- High-resolution spatial imaging mass cytometry on tissue sections (FFPE and snap-frozen).
Publications
Venegoni, C.; Raineri, D.; Mazzucca, C. B.; Ghazanfar, A.; Cappellano, G.; Baricich, A.; Patrucco, F.; Zeppegno, P.; Gramaglia, C.; Balbo, P. E.; Cantaluppi, V.; Patti, G.; Giordano, M.; Manfredi, M.; Rolla, R.; Sainaghi, P. P.; Pirisi, M.; Bellan, M.; Chiocchetti, A. Post-COVID-19 Sequelae Are Associated with Sustained SARS-CoV-2-Specific CD4+ Immune Responses. Int. Immunopharmacol. 2025, 148, 114103. https://doi.org/10.1016/j.intimp.2025.114103.
Stolfi, F.; Brasso, C.; Raineri, D.; Landra, V.; Mazzucca, C. B.; Ghazanfar, A.; Scotti, L.; Sinella, R.; Villari, V.; Cappellano, G.; Rocca, P.; Chiocchetti, A. Deep Immunophenotyping of Circulating Immune Cells in Major Depressive Disorder Patients Reveals Immune Correlates of Clinical Course and Treatment Response. Brain Behav. Immun. – Health 2025, 43, 100942. https://doi.org/10.1016/j.bbih.2024.100942.
Raineri, D.; Mazzucca, C. B.; Moia, R.; Bruna, R.; Kustrimovic, N.; Cappellano, G.; Bellan, M.; Perazzi, M.; Gaidano, G.; Chiocchetti, A. Impairment of the T Cell Memory Response in Chronic Lymphocytic Leukemia Patients after SARS-CoV-2 Vaccination. Vaccine 2025, 48, 126723. https://doi.org/10.1016/j.vaccine.2025.126723.
Raineri, D.; Savastio, S.; Bellone, S.; Scotti, L.; Barbero-Mazzucca, C.; Cappellano, G.; Prodam, F.; Pozzi, E.; Rabbone, I.; Chiocchetti, A. Memory Regulatory T Cells as a Biomarker of Early Type 1 Diabetes. Diabetes 2026, 75 (4), 676–682. https://doi.org/10.2337/db25-0161.
CONTACTS
For any information, please contact
corefacilities.ipazia@uniupo.it
For any information, please contact
corefacilities.ipazia@uniupo.it
or segreteria.caad@uniupo.
CAAD
Center for Translational Research on
Autoimmune and Allergic Disease
Centro di Ricerca Traslazionale
sulle Malattie Autoimmuni e Allergiche
Corso Trieste 15/A
Novara – 28100 (ITALY)
Next-Gen Flow
Cytometry & Sorting
Next-Gen flow
Cytometry & Sorting
The Next-Gen flow Cytometry & Sorting Core Facility provides advanced services in multiparametric flow cytometry and mass cytometry, supporting experimental design, antibody panel development, training, and data analysis.
Its mission is to enable cutting-edge, high-dimensional single-cell analysis to foster translational and precision research.
Technical
Expert
Dr. Gloria Olearo
INSTRUMENTS
The facility is equipped with:
- BD FACSymphony™ A5: High-parameter cytometer (5 lasers, 31 detectors) for up to 29-color analysis.
- BD FACSCanto™ II: User-friendly analyzer with HTS option for high-throughput analysis (3 lasers, 10 detectors).
- BD FACSAria™ Fusion: High-performance, biosafety-equipped cell sorter (4-way, 14-color sorting).
- BD Rhapsody™ System: Single-cell capture and barcoding platform for transcriptomic and proteomic profiling.
- Helios™ Mass Cytometer: High-dimensional single-cell analysis using metal-tagged antibodies (up to 45 markers).
- Hyperion™ Imaging System: Mass cytometry-based imaging for spatial profiling in tissues (>1 mm² scan area, >40 markers).
Reserve instruments
To reserve instruments, use the “Agendo” management system: https://upo.agendoscience.com/.
Infrastucture units
The Core Facility is composed of two Infrastructure Units:
1. Multiparametric flow cytometry with cell sorting capabilities and Rhapsody system for single-cell RNA sequencing
The multiparametric flow cytometry unit with cell sorting capabilities enables rapid and precise analysis (via 2 analyzers) and isolation (via 1 cell sorter) of cellular subpopulations. The Rhapsody system for single-cell RNA sequencing allows for the characterization of the transcriptomic profile of individual cells, integrating it with various surface markers through the use of oligonucleotide-conjugated antibodies. This provides unprecedented resolution in the study of cellular heterogeneity.
Scientific supervisor: Dr. Davide Raineri
The Unit offers:
- Multiparametric analysis of up to 30 cellular markers simultaneously.
- Cell sorting (up to 4 populations simultaneously, including plate-based sorting).
- scRNA-seq library preparation using BD Rhapsody technology.
2. Mass cytometry (CyTOF) combined with Hyperion imaging
The mass cytometry unit enables the simultaneous analysis of over 40 parameters in single-cell suspensions. In addition, integration with the Hyperion imaging mass cytometry system allows a high-resolution spatial exploration of tissue architecture, supporting advanced studies of the tissue microenvironment and cell interactions.
Scientific supervisor: Prof. Annalisa Chiocchetti
The Unit offers:
- Cytometric analysis of cell suspensions with over 40 markers.
- High-resolution spatial imaging mass cytometry on tissue sections (FFPE and snap-frozen).
Publications
Venegoni, C.; Raineri, D.; Mazzucca, C. B.; Ghazanfar, A.; Cappellano, G.; Baricich, A.; Patrucco, F.; Zeppegno, P.; Gramaglia, C.; Balbo, P. E.; Cantaluppi, V.; Patti, G.; Giordano, M.; Manfredi, M.; Rolla, R.; Sainaghi, P. P.; Pirisi, M.; Bellan, M.; Chiocchetti, A. Post-COVID-19 Sequelae Are Associated with Sustained SARS-CoV-2-Specific CD4+ Immune Responses. Int. Immunopharmacol. 2025, 148, 114103. https://doi.org/10.1016/j.intimp.2025.114103.
Stolfi, F.; Brasso, C.; Raineri, D.; Landra, V.; Mazzucca, C. B.; Ghazanfar, A.; Scotti, L.; Sinella, R.; Villari, V.; Cappellano, G.; Rocca, P.; Chiocchetti, A. Deep Immunophenotyping of Circulating Immune Cells in Major Depressive Disorder Patients Reveals Immune Correlates of Clinical Course and Treatment Response. Brain Behav. Immun. – Health 2025, 43, 100942. https://doi.org/10.1016/j.bbih.2024.100942.
Raineri, D.; Mazzucca, C. B.; Moia, R.; Bruna, R.; Kustrimovic, N.; Cappellano, G.; Bellan, M.; Perazzi, M.; Gaidano, G.; Chiocchetti, A. Impairment of the T Cell Memory Response in Chronic Lymphocytic Leukemia Patients after SARS-CoV-2 Vaccination. Vaccine 2025, 48, 126723. https://doi.org/10.1016/j.vaccine.2025.126723.
Raineri, D.; Savastio, S.; Bellone, S.; Scotti, L.; Barbero-Mazzucca, C.; Cappellano, G.; Prodam, F.; Pozzi, E.; Rabbone, I.; Chiocchetti, A. Memory Regulatory T Cells as a Biomarker of Early Type 1 Diabetes. Diabetes 2026, 75 (4), 676–682. https://doi.org/10.2337/db25-0161.
CONTACTS
For any information, please contact
corefacilities.ipazia@uniupo.it
For any information, please contact
corefacilities.ipazia@uniupo.it
or segreteria.caad@uniupo.
CAAD
Center for Translational Research on
Autoimmune and Allergic Disease
Centro di Ricerca Traslazionale
sulle Malattie Autoimmuni e Allergiche
Corso Trieste 15/A
Novara – 28100 (ITALY)